other:Article Title: Targeting of a novel interplay between MET tyrosine kinase and NRF2 enhances sensitivity to Paclitaxel in triple negative breast cancer
Article Snippet: Primary antibodies are used as follows: anti-NRF2 (D1Z9C) (12721; Cell Signalling Technology), anti-NRF2 (EP1808Y) (ab-62352; Abcam), anti-phospho-SRC (Tyr416) (2101; Cell Signalling Technology), anti-SRC (2108; Cell Signalling Technology), anti-phospho-MET (Tyr1234/35) (3126; Cell Signalling Technology), anti-MET (3127; Cell Signalling Technology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-phospho-histone H2AX (Ser139) (9718; Cell Signalling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-KEAP1 (F-10) (sc-514914; Santa Cruz Biotechnology), anti-p62 (SQSTM1) (PM045; MBL International), anti-phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-phospho-EGFR (Tyr1068) (3777; Cell Signalling Technology), anti-EGFR (LA22) (05–104, Sigma-Aldrich), anti-8-OHdG (sc-66036, Santa Cruz Biotechnology), anti-vinculin (13901; Cell Signalling Technology), anti-lamin A/C (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (sc-47724; Santa Cruz Biotechnology), anti-β-Actin (3700, Cell Signalling Technology); PHA-665,752 (S1070; TargetMol), Dasatinib (CDS023389; Sigma-Aldrich), ML-385 (S8790; Selleckchem), Paclitaxel (T7191; Sigma-Aldrich);
Fractionation:Article Title: Targeting of a novel interplay between MET tyrosine kinase and NRF2 enhances sensitivity to Paclitaxel in triple negative breast cancer.
Article Snippet: .. Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (12721; Cell Signalling Technology), anti-NRF2 (EP1808Y) (ab-62352; Abcam), anti-phospho-SRC (Tyr416) (2101; Cell Signalling Technology), anti-SRC (2108; Cell Signalling Technology), anti-phospho-MET (Tyr1234/35) (3126; Cell Signalling Technology), anti-MET (3127; Cell Signalling Technology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-phospho-histone H2AX (Ser139) (9718; Cell Signalling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-KEAP1 (F10) (sc-514914; Santa Cruz Biotechnology), anti-p62 (SQSTM1) (PM045; MBL International), anti- AR TIC LE IN PR ES S phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-phospho-EGFR (Tyr1068) (3777; Cell Signalling Technology), anti-EGFR (LA22) (05-104, Sigma-Aldrich), anti-8-OHdG (sc66036, Santa Cruz Biotechnology), anti-vinculin (13901; Cell Signalling Technology), anti-lamin A/C (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (sc-47724; Santa Cruz Biotechnology), anti-β-Actin (3700, Cell Signalling Technology); PHA-665752 (S1070; TargetMol), Dasatinib (CDS023389; Sigma-Aldrich), ML-385 (S8790; Selleckchem), Paclitaxel (T7191; Sigma-Aldrich); Protein extract, nuclei/cytoplasm fractionation and western blot analyses Total protein lysates were prepared using Buffer A (10 mM Hepes [pH 7.9], 10 mM KCl, 1.5 mM MgCl2, 0.5 mM DTT, 0.1% NP-40) or RIPA Buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP40, 12mM sodium deoxycholate) supplemented with 10 mg/ml Protease Inhibitor Cocktail-1 (P2714; Sigma-Aldrich), 10 mg/mL TPCK, 1mM phenylmethylsulfonyl fluoride, 25mM NaF, 1mM sodium orthovanadate, 25 mM β-glycerophosphate. ..
Western Blot:Article Title: Targeting of a novel interplay between MET tyrosine kinase and NRF2 enhances sensitivity to Paclitaxel in triple negative breast cancer.
Article Snippet: .. Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (12721; Cell Signalling Technology), anti-NRF2 (EP1808Y) (ab-62352; Abcam), anti-phospho-SRC (Tyr416) (2101; Cell Signalling Technology), anti-SRC (2108; Cell Signalling Technology), anti-phospho-MET (Tyr1234/35) (3126; Cell Signalling Technology), anti-MET (3127; Cell Signalling Technology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-phospho-histone H2AX (Ser139) (9718; Cell Signalling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-KEAP1 (F10) (sc-514914; Santa Cruz Biotechnology), anti-p62 (SQSTM1) (PM045; MBL International), anti- AR TIC LE IN PR ES S phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-phospho-EGFR (Tyr1068) (3777; Cell Signalling Technology), anti-EGFR (LA22) (05-104, Sigma-Aldrich), anti-8-OHdG (sc66036, Santa Cruz Biotechnology), anti-vinculin (13901; Cell Signalling Technology), anti-lamin A/C (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (sc-47724; Santa Cruz Biotechnology), anti-β-Actin (3700, Cell Signalling Technology); PHA-665752 (S1070; TargetMol), Dasatinib (CDS023389; Sigma-Aldrich), ML-385 (S8790; Selleckchem), Paclitaxel (T7191; Sigma-Aldrich); Protein extract, nuclei/cytoplasm fractionation and western blot analyses Total protein lysates were prepared using Buffer A (10 mM Hepes [pH 7.9], 10 mM KCl, 1.5 mM MgCl2, 0.5 mM DTT, 0.1% NP-40) or RIPA Buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP40, 12mM sodium deoxycholate) supplemented with 10 mg/ml Protease Inhibitor Cocktail-1 (P2714; Sigma-Aldrich), 10 mg/mL TPCK, 1mM phenylmethylsulfonyl fluoride, 25mM NaF, 1mM sodium orthovanadate, 25 mM β-glycerophosphate. ..
Article Title: Application of a novel myristoylproteomics approach identifies GLIPR2 as a key pro-ferroptotic substrate in non-small cell lung cancer.
Article Snippet: .. The primary antibodies used for SDS-PAGE and western blot were as follows: anti-NMT1 (abcam, #ab186123; 1:1000, Cambridge, Cambs, UK), antiNMT2 (abcam, #ab224045; 1:1000), anti-ACTB (Servicebio, #GB12001-100; 1:3000, Wuhan, Hubei, China), anti-GAPDH (Servicebio, #GB12002-100; 1:3000), anti-TAMRA (abcam, #ab171120; 1:1000), anti-PRKACA (Cell Signaling Technology, #5842S; 1:500, Danvers, MA, USA), anti-SRC (Cell Signaling Technology, #2123; 1:500), anti-CHCHD3 (abcam, #ab224565; 1:1000), anti-FSP1 (SANTA CRUZ, #sc-377120; 1:1000, Dallas, TX, USA), anti-GLIPR2 (SANTA CRUZ, #sc-398529; 1:500), anti-GLIPR2 (abcam, #ab122059; 1:250), anti-OCC1 (Thermo Scientific, #PA5-20673; 1:500, Waltham, MA, USA), anti-RFTN1 (Proteintech, 24289-1-AP; 1:2000, Chicago, IL, USA), and anti-FLAG (Thermo Scientific, #MA1-91878; 1:1000), antiSLC7A11 (ABclonal, A2413, 1:2000, Wuhan, China), anti-LPCAT3 (Proteintech, 67882-1-Ig, 1:1000), anti-ACSL4 (Proteintech, 22401-1- AP, 1:5000), anti-GPX4 (Proteintech, 67763-1-Ig, 1:2000), anti-FTH (ABclonal, A1144, 1:1000), anti-FTL (ABclonal, A1768, 1:1000), antiTFRC (Proteintech, 84766-4-RR, 1:2000). .. The primary antibodies used for IHC were anti‐4‐HNE antibody (abcam, #48506).
Article Title: Application of a novel myristoylproteomics approach identifies GLIPR2 as a key pro-ferroptotic substrate in non-small cell lung cancer
Article Snippet: .. The primary antibodies used for SDS-PAGE and western blot were as follows: anti-NMT1 (abcam, #ab186123; 1:1000, Cambridge, Cambs, UK), anti-NMT2 (abcam, #ab224045; 1:1000), anti-ACTB (Servicebio, #GB12001-100; 1:3000, Wuhan, Hubei, China), anti-GAPDH (Servicebio, #GB12002-100; 1:3000), anti-TAMRA (abcam, #ab171120; 1:1000), anti-PRKACA (Cell Signaling Technology, #5842S; 1:500, Danvers, MA, USA), anti-SRC (Cell Signaling Technology, #2123; 1:500), anti-CHCHD3 (abcam, #ab224565; 1:1000), anti-FSP1 (SANTA CRUZ, #sc-377120; 1:1000, Dallas, TX, USA), anti-GLIPR2 (SANTA CRUZ, #sc-398529; 1:500), anti-GLIPR2 (abcam, #ab122059; 1:250), anti-OCC1 (Thermo Scientific, #PA5-20673; 1:500, Waltham, MA, USA), anti-RFTN1 (Proteintech, 24289-1-AP; 1:2000, Chicago, IL, USA), and anti-FLAG (Thermo Scientific, #MA1-91878; 1:1000), anti-SLC7A11 (ABclonal, A2413, 1:2000, Wuhan, China), anti-LPCAT3 (Proteintech, 67882-1-Ig, 1:1000), anti-ACSL4 (Proteintech, 22401-1-AP, 1:5000), anti-GPX4 (Proteintech, 67763-1-Ig, 1:2000), anti-FTH (ABclonal, A1144, 1:1000), anti-FTL (ABclonal, A1768, 1:1000), anti-TFRC (Proteintech, 84766-4-RR, 1:2000). .. The primary antibodies used for IHC were anti‐4‐HNE antibody (abcam, #48506).
Protease Inhibitor:Article Title: Targeting of a novel interplay between MET tyrosine kinase and NRF2 enhances sensitivity to Paclitaxel in triple negative breast cancer.
Article Snippet: .. Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (12721; Cell Signalling Technology), anti-NRF2 (EP1808Y) (ab-62352; Abcam), anti-phospho-SRC (Tyr416) (2101; Cell Signalling Technology), anti-SRC (2108; Cell Signalling Technology), anti-phospho-MET (Tyr1234/35) (3126; Cell Signalling Technology), anti-MET (3127; Cell Signalling Technology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-phospho-histone H2AX (Ser139) (9718; Cell Signalling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-KEAP1 (F10) (sc-514914; Santa Cruz Biotechnology), anti-p62 (SQSTM1) (PM045; MBL International), anti- AR TIC LE IN PR ES S phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-phospho-EGFR (Tyr1068) (3777; Cell Signalling Technology), anti-EGFR (LA22) (05-104, Sigma-Aldrich), anti-8-OHdG (sc66036, Santa Cruz Biotechnology), anti-vinculin (13901; Cell Signalling Technology), anti-lamin A/C (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (sc-47724; Santa Cruz Biotechnology), anti-β-Actin (3700, Cell Signalling Technology); PHA-665752 (S1070; TargetMol), Dasatinib (CDS023389; Sigma-Aldrich), ML-385 (S8790; Selleckchem), Paclitaxel (T7191; Sigma-Aldrich); Protein extract, nuclei/cytoplasm fractionation and western blot analyses Total protein lysates were prepared using Buffer A (10 mM Hepes [pH 7.9], 10 mM KCl, 1.5 mM MgCl2, 0.5 mM DTT, 0.1% NP-40) or RIPA Buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP40, 12mM sodium deoxycholate) supplemented with 10 mg/ml Protease Inhibitor Cocktail-1 (P2714; Sigma-Aldrich), 10 mg/mL TPCK, 1mM phenylmethylsulfonyl fluoride, 25mM NaF, 1mM sodium orthovanadate, 25 mM β-glycerophosphate. ..
Bioprocessing:Article Title: Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cells.
Article Snippet: The membranes were incubated with anti-Flotillin (Abcam Cambridge, UK; ab13493) anti-E-cadherin (GT311 GeneTex, Irvine, California, USA), anti-vimentin (M0725, Dako, Glostrup, Denmark), anti-phospho-Fibroblast Growth Factor Receptor Substrate 2 α (FRS2-α) (Tyr196) (Cell Signaling Technology, Beverly, MA, USA, 3864), the anti-phospho-Sarcoma kinase (Src) Family (Tyr416, D49G4) (Cell Signaling Technology, Beverly, MA; USA;6943), monoclonal antibodies anti-pMTOR (Ser 2448; Cell Signaling; Beverly, MA, USA; 5536S), anti-p-p44/42 mitogen-activated protein kinase (MAPK) (p-ERK1/2) (Thr202/Tyr204; Cell Signaling; 9101S), anti-Bek (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; C17, sc-122), anti-p-S6K (ser 371, Cell Signaling, Beverly, MA, USA; 9208), and anti p-PKCε (Ser729, Abcam, Cambridge, UK; ab63387), anti-Myeloid Cell Leukemia 1 (Mcl-1) (D35A5) (Cell Signaling; Beverly, MA, USA; 5453), anti-TRPA1 (Abcam; Abcam Cambridge, UK; ab62053),. .. The membranes were stripped as reported [6] and probed again with anti-p44/42 MAPK (ERK1/2) (Cell Signaling; Beverly, MA, USA; 4695S), anti-S6K (Cell Signaling; Beverly, MA, USA;9202), anti-PKCε (Abcam; Abcam Cambridge, UK;ab124806), anti-FRS2 (H-91) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; sc-8318), anti-HSP90 (Proteintech Inc., Rosemont, IL, USA, 13171-1-AP), antiMTOR (Cell Signaling; Beverly, MA, USA; 2983S) polyclonal antibodies or anti-Src (Cell Signaling; Beverly, MA, USA;36D10), anti-ACTB (Sigma-Aldrich; St. Louis, Missouri, USA; A5441) monoclonal antibodies for protein equal loading. ..
SDS Page:Article Title: Application of a novel myristoylproteomics approach identifies GLIPR2 as a key pro-ferroptotic substrate in non-small cell lung cancer.
Article Snippet: .. The primary antibodies used for SDS-PAGE and western blot were as follows: anti-NMT1 (abcam, #ab186123; 1:1000, Cambridge, Cambs, UK), antiNMT2 (abcam, #ab224045; 1:1000), anti-ACTB (Servicebio, #GB12001-100; 1:3000, Wuhan, Hubei, China), anti-GAPDH (Servicebio, #GB12002-100; 1:3000), anti-TAMRA (abcam, #ab171120; 1:1000), anti-PRKACA (Cell Signaling Technology, #5842S; 1:500, Danvers, MA, USA), anti-SRC (Cell Signaling Technology, #2123; 1:500), anti-CHCHD3 (abcam, #ab224565; 1:1000), anti-FSP1 (SANTA CRUZ, #sc-377120; 1:1000, Dallas, TX, USA), anti-GLIPR2 (SANTA CRUZ, #sc-398529; 1:500), anti-GLIPR2 (abcam, #ab122059; 1:250), anti-OCC1 (Thermo Scientific, #PA5-20673; 1:500, Waltham, MA, USA), anti-RFTN1 (Proteintech, 24289-1-AP; 1:2000, Chicago, IL, USA), and anti-FLAG (Thermo Scientific, #MA1-91878; 1:1000), antiSLC7A11 (ABclonal, A2413, 1:2000, Wuhan, China), anti-LPCAT3 (Proteintech, 67882-1-Ig, 1:1000), anti-ACSL4 (Proteintech, 22401-1- AP, 1:5000), anti-GPX4 (Proteintech, 67763-1-Ig, 1:2000), anti-FTH (ABclonal, A1144, 1:1000), anti-FTL (ABclonal, A1768, 1:1000), antiTFRC (Proteintech, 84766-4-RR, 1:2000). .. The primary antibodies used for IHC were anti‐4‐HNE antibody (abcam, #48506).
Article Title: Application of a novel myristoylproteomics approach identifies GLIPR2 as a key pro-ferroptotic substrate in non-small cell lung cancer
Article Snippet: .. The primary antibodies used for SDS-PAGE and western blot were as follows: anti-NMT1 (abcam, #ab186123; 1:1000, Cambridge, Cambs, UK), anti-NMT2 (abcam, #ab224045; 1:1000), anti-ACTB (Servicebio, #GB12001-100; 1:3000, Wuhan, Hubei, China), anti-GAPDH (Servicebio, #GB12002-100; 1:3000), anti-TAMRA (abcam, #ab171120; 1:1000), anti-PRKACA (Cell Signaling Technology, #5842S; 1:500, Danvers, MA, USA), anti-SRC (Cell Signaling Technology, #2123; 1:500), anti-CHCHD3 (abcam, #ab224565; 1:1000), anti-FSP1 (SANTA CRUZ, #sc-377120; 1:1000, Dallas, TX, USA), anti-GLIPR2 (SANTA CRUZ, #sc-398529; 1:500), anti-GLIPR2 (abcam, #ab122059; 1:250), anti-OCC1 (Thermo Scientific, #PA5-20673; 1:500, Waltham, MA, USA), anti-RFTN1 (Proteintech, 24289-1-AP; 1:2000, Chicago, IL, USA), and anti-FLAG (Thermo Scientific, #MA1-91878; 1:1000), anti-SLC7A11 (ABclonal, A2413, 1:2000, Wuhan, China), anti-LPCAT3 (Proteintech, 67882-1-Ig, 1:1000), anti-ACSL4 (Proteintech, 22401-1-AP, 1:5000), anti-GPX4 (Proteintech, 67763-1-Ig, 1:2000), anti-FTH (ABclonal, A1144, 1:1000), anti-FTL (ABclonal, A1768, 1:1000), anti-TFRC (Proteintech, 84766-4-RR, 1:2000). .. The primary antibodies used for IHC were anti‐4‐HNE antibody (abcam, #48506).
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